| 摘要: |
| 目的:构建一种可用于卵巢癌诊断和术前治疗的核酸嵌合体。方法:设计一种癌抗原125（CA125）-Plk1嵌合体,培养OVCAR-3卵巢癌细胞,平均分成嵌合体组（加入1μl的CA125-Plk1嵌合体）、抗体组（加入1μl的CA125抗体）、对照组（加入1μl的0.9%氯化钠液）。流式细胞仪检测3组结合卵巢癌细胞能力;噻唑蓝（MTT）增殖实验检测3组卵巢癌细胞增殖的情况;Tunel染色检测嵌合体组和对照组卵巢癌细胞凋亡的情况。结果:流式细胞检测结果示,嵌合体组中CA125-Plk1嵌合体结合卵巢癌细胞的比例（83.67%）高于抗体组中CA125抗体与卵巢癌细胞结合比例（65.12%）和对照组与卵巢癌细胞结合比例（30.74%）,差异有统计学意义（P<0.05）;MTT检测结果显示,嵌合体组卵巢癌细胞的增殖率（32.60%）低于对照组（62.24%）和抗体组（57.99%）,差异均有统计学意义（P<0.05）;Tunel染色结果显示,嵌合体组卵巢癌细胞中可以见到SiRNA荧光表达,与对照组比较,嵌合体组卵巢癌细胞凋亡比例明显上升[（78.46±6.33）%vs（19.73±4.27）%],差异有统计学意义（P<0.05）。结论:CA125-Plk1嵌合体能特异性地结合卵巢癌细胞,通过抑制增殖和促凋亡,达到一个潜在的诊断和术前治疗作用。 |
| 关键词: 卵巢癌 适配体 增殖 凋亡 |
| DOI： |
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| Construction of Nucleic Acid Chimera for Diagnosis and Preoperative Treatment of Ovarian Cancer |
| DONG Li;WANG Yue;WANG Zhenhua |
| (Henan Provincial People’s Hospital) |
| Abstract: |
| Objective: To construct one nucleic acid chimera for diagnosis and preoperative treatment of ovarian cancer.Methods: Culture of OVCAR-3 ovarian cancer cells were divided into chimerism group,antibody group and control group.A CA125-Plk1 chimera was designed and PEG was used to increase the stability of nucleic acid chimera; flow cytometry method was used to detect ability of chimerism combining with ovarian cancer cells; MTT was used to detect the influence of chimerism on proliferation of ovarian cancer cells; Tunel staining was used to detect the effect of chimerism on ovarian cancer cell apoptosis. Results: Flow cytometry showed that the chimera group（ 83. 67%） with ovarian cancer cells was higher than that of antibody group（ 65. 12%） and control group（ 30. 74%）,the difference was statistically significant（ P<0. 05）; The results of MTT showed that the proliferation rate of ovarian cancer cells in the chimera group was（ 32. 6%）,which was decreased by（ 62. 24%） and（ 57. 99%）,respectively（ P<0. 05）; Tunel staining showed that compared with control group[（ 19. 73±4. 27） %],the ovarian cancer cell apoptosis ratio in chimera group[（ 78. 46±6. 33） %]increased significantly（ P<0. 05）.Conclusions: We constructed a CA125-Plk1 chimera,which can specifically bind ovarian cancer cells,inhibit proliferation and promote apoptosis,reaching a potential diagnostic and preoperative therapeutic effect. |
| Key words: Ovariancancer Aptamer Proliferation Apoptosis |
