| 摘要: |
| 目的:寻找胎鼠宫内脑损伤的有效预测指标。方法:比较正常对照组和缺氧组胎鼠脑组织形态学变化、脑组织中S100B蛋白和脑源性神经营养因子(BDNF)的表达及含量。结果:(1)脑组织形态学结果显示:缺氧组胎鼠大脑皮质和海马区细胞排列紊乱,细胞数目减少,结构模糊或消失,组织间质疏松水肿。(2)免疫组织化学染色结果显示:S100B蛋白主要在海马区的齿状回部位表达,BDNF主要在海马区和皮质区表达。缺氧组S100B蛋白及BDNF阳性细胞平均灰度值较正常对照组明显增强(189.90±31.66 vs 84.66±12.90,P=0.000;139.04±15.83 vs 74.86±14.08,P=0.000)。(3)酶联免疫吸附试验检测结果显示:缺氧组胎鼠脑组织中S100B蛋白、BDNF含量均明显高于正常对照组(3.17±1.07 ng/ml vs 2.25±1.20 ng/ml,P=0.006;152.08±36.29 pg/ml vs 128.21±43.43 pg/ml,P=0.039)。结论:孕鼠缺氧后可导致胎鼠脑损伤,脑组织中S100B蛋白、BDNF的测定可作为预测胎鼠宫内脑损伤的指标。 |
| 关键词: S100B蛋白 脑源性神经营养因子 胎鼠宫内脑损伤 |
| DOI： |
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| 基金项目: |
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| The Predictive Value of S100B Protein and BDNF in Brain Tissue for Fetal Rats Intrauterine Brain Injury |
| GUO Xiaoyue;SHAO Hui;WANG Yan;ZHAO Yangyu; |
| (Peking University Third Hospital) |
| Abstract: |
| Objective: Looking for effective predictors of fetal rats’ brain injury. Methods: Observe the morphologic change of fetal rats’ brain tissues,and the expression level of S100 B protein and brain derived neurotrophic factor in brain tissues. Results:(1)After hypoxic cerebral,the cortex and hippocampus cells arranged disorder,the number of cells were reduced,the structure was fuzzy or disappeared,and the tissue stroma was edema.(2)Immunohistochemical staining results suggested S100 B protein is mainly expressed in the dentate gyrus of hippocampus,BDNF is mainly expressed in the hippocampus and cortical regions. The expression of S100 B protein and BDNF positive cells in hypoxia group was obviously increased compared with control group( 189. 90 ± 31. 66 vs 84. 66 ±12. 90; 139. 04±15. 83 vs 74. 86 ±14. 08),differences were statistically significant( P = 0. 000).(3) Enzyme-linked immunosorbent assay results showed that the content of S100 B protein and BDNF in fetal rats’ brain tissues in hypoxia group were significantly higher than in control group( 3. 17 ±1. 07 ng/ml vs 2. 25 ±1. 20 ng/ml; 152. 08 ±36. 29 pg/ml vs 128. 21 ±43. 43 pg/ml),differences were statistically significant( P = 0. 006,P = 0. 039). Conclusions: Fetal rats’ brain injury occurred when pregnant rat experienced hypoxia. The detection of S100 B and BDNF in brain tissues can be used to predict fetal rats’ intrauterine brain injury. |
| Key words: S100Bprotein Brainderivedneurophicfactor Intrauterinebraininjuryoffetalrat |
