| 摘要: |
| 目的:探究P57KIP2基因过表达之后对滋养层细胞生物学行为的影响。方法:采用实时荧光定量(q PCR)及Western Blot方法检测人绒毛膜滋养层细胞(HTR8-/SVneo)过表达P57KIP2基因后mRNA和蛋白水平的表达变化; 用Transwell方法检测HTR8-/SVneo细胞过表达P57KIP2基因后迁移、侵袭能力生物学行为的变化; CCK8检测HTR8-/SVneo细胞过表达该基因后增殖能力的变化。采用免疫荧光检测HTR8-/SVneo细胞过表达该基因后,抗凋亡蛋白Bcl2的表达变化。结果:滋养层细胞过表达P57KIP2基因后,其mRNA及蛋白水平均明显增高(P<0.05); P57KIP2基因过表达后滋养层细胞的迁移、侵袭数量均明显增高,差异有统计学意义(P<0.05); 滋养层细胞的细胞相对吸光值48小时后明显增高(P<0.001); 过表达P57KIP2基因后滋养层细胞中抗凋亡蛋白Bcl2表达增高(P<0.05)。结论:P57KIP2基因可能会增加滋养层细胞迁移、侵袭以及增殖能力,P57KIP2基因可能参与调控滋养细胞的凋亡过程。 |
| 关键词: 子痫前期 P57KIP2基因 迁移 侵袭 凋亡 |
| DOI： |
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| 基金项目:国家自然科学基金项目(编号:81471463);四川省科技厅课题(编号:2014JY0158) |
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| Overexpression P57KIP2 Gene Affects Human Extravillous Trophoblast Cell Behaviors |
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| Abstract: |
| Objective:To check the effect P57KIP2 overexpression on biological behaviors of trophoblast cell.Methods:After P57KIP2 overexpression,the mRNA and protein expression of P57KIP2 were detected by realtime fluorescence quantitative(q PCR) and Western Blot to detectin human trophoblast cell(HTR8-/SVneo).The change of migration and invasion were test by Transwell method,the changes the proliferation was detected by CCK8;the change of the expression of anti-apoptotic protein Bcl2 was detected by immunofluorescence staining.Results:Overexpresion P57KIP2 significantly increased the relative mRNA and protein expression of P57KIP2gene(P<0.05);and the number of imgration and invasion of trophoblast cells were significantly increased(P <0.05);and the proliferation of trophoblast cells was significantly enhanced after 48 hours(P<0.001);the expression of antiapoptotic protein Bcl2 was increased in human trophoblast cells(P<0.05).Conclusions:P57KIP2 gene may increase the ability of migration,invasion and proliferation of trophoblast cells.P57KIP2 gene may be involved in the regulation of apoptosis of trophoblast cell. |
| Key words: Preeclampsia P57KIP2 Migration Invasion Apoptosis |
