| 摘要: |
| 目的:研究雌激素受体β基因（ESR2）的多态性与不明原因复发性流产（URSA）之间的关系。方法:选择2010年8月至2015年5月我院收治的URSA女性85例作为研究组,另根据研究组受试者的年龄、体质量指数（BMI）、学历等基线资料匹配选择85例我院体检健康的有2次及以上的成功怀孕且活产的非妊娠妇女作为对照组。在月经周期的23天取各受试者的肘静脉血5 ml,比较两组妇女卵泡刺激素（FSH）、黄体生成激素（LH）和雌二醇（E2）的血清水平,同时检测ESR2基因的rs1256049、rs4986938和rs1256030位点多态性,分析ESR2各基因位点多态性及其与激素水平间的关系。结果:两组妇女FSH和E2水平差异无统计学意义（P>0.05）。研究组妇女LH水平明显高于对照组,差异有统计学意义（P<0.05）。两组妇女的rs1256049、rs4986938、rs1256030位点的各基因型频率及不同位点与各基因型（野生型、杂合型、纯合型）血清FSH、E2和LH的水平间比较,差异均无统计学意义（P>0.05）。对照组妇女中仅rs4986938位点与rs1256030位点多态性呈正相关（r=0.38,P=0.00）,研究组妇女中仅rs1256049位点与rs4986938位点的多态性之间呈负相关（r=-0.17,P=0.02）。结论:ESR2的多态性与URSA之间无明显关系。三个位点间有一定的相关性,且在两组中是不同的,这种差异是否与URSA间存在一定的联系,还需要进一步研究。 |
| 关键词: 雌激素受体β基因 习惯性流产 限制性片段长度多态性 单核苷酸多态性 |
| DOI： |
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| 基金项目: |
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| Study on the Relationship Between Estrogen Receptor β Gene Polymorphism and Unexplained Recurrent Spontaneous Abortion |
| GUO Chunyan;AI Hongmei |
| (Chongqing Emergency Medical Center;Department of Orthopedic Surgery,General Hospital of Coal Industry Ministry of China) |
| Abstract: |
| Objective: To study the relationship between polymorphism of estrogen receptor β gene( ESR2)and unexplained recurrent spontaneous abortion( URSA).Methods: A total of 85 women with recurrent spontaneous abortion were recruited in our hospital from August 2010 to May 2015.A total of 85 healthy ethnically matched women with two or more successful pregnancies and live births and no history of complicated pregnancies were recruited as the control group.5 ml elbow vein blood was extracted in the menstrual cycle of 2-3 days,and serum levels of follicle stimulating hormone( FSH),luteinizing hormone( LH),and estradiol( E2) were measured and compared between two groups,the rs1256049,rs4986938 and rs1256030 polymorphisms of ESR2 gene were detected and the correlation between ESR2 gene locus polymorphism and URSA was analyzed.Results: There was no significant difference in FSH and E2 level between the two groups( P>0. 05).The level of LH in the study group was significantly higher than that in the control group( P < 0. 05). There were no significant differences in the rs1256049 locus,rs4986938 locus and rs1256030 locus between the two groups.The level of FSH,LH and E2 in rs1256049 locus,rs4986938 locus,rs1256030 locus of all genotypes of females were not statistically significant( P>0. 05).There was a negative correlation between the polymorphism of rs1256049 locus and the rs4986938 locus among all subjects,and rs4986938 locus were positively correlated with rs1256030 polymorphism.There was a positive correlation between the rs4986938 locus and rs1256030 locus polymorphism( r = 0. 38,P = 0. 00) in the control group,and there was a negative correlation between the rs1256049 locus and the polymorphism of the rs4986938 locus in the study group( r =-0. 17,P = 0. 02). Conclusions: There is no significant relationship between ESR2 polymorphism and URSA. There is a certain correlation between the three mutation sites of ESR2 gene,and this correlation is different between the two groups,but whether there is a link between URSA and this difference still needs further study |
| Key words: Estrogenreceptorβgene Habitualabortion Restrictionfragmentlengthpolymorphism Singlenucleotidepolymorphism |
